Journal: Cancers

Article Title: Whole Genome Sequencing of Spontaneously Occurring Rat Natural Killer Large Granular Lymphocyte Leukemia Identifies JAK1 Somatic Activating Mutation

doi: 10.3390/cancers12010126

Figure Lengend Snippet: Cancer Gene Census (CGC) query revealed genes mutated in the RNK-16 cell line that are implicated in cancer. Tier levels are assigned by CGC and indicate a depth of evidence of mutations in those genes having oncogenic transformative potential. Variants in Ddr 2, Fat 1, Jak 1, Ncor 2, and Stag were confirmed using Sanger sequencing in the RNK-16 cell line. Only the mutation in Jak 1 was confirmed in the RNK-16 primary spleen cells.

Article Snippet: Table S1: Alignment statistics for the RNK-16 and F344/NCrl samples, Table S2: Complete list of variants with an alternate allele in the RNK-16 cell line compared to F344/NCrl, Table S3: List of human homolog genes mapped to rat genes, with variants in RNK-16 cell line filtered by Q score and impact factor, Table S4: Primer design for WGS validation by Sanger sequencing.

Techniques: Sequencing, Mutagenesis, Biomarker Discovery

Journal: Cancers

Article Title: Whole Genome Sequencing of Spontaneously Occurring Rat Natural Killer Large Granular Lymphocyte Leukemia Identifies JAK1 Somatic Activating Mutation

doi: 10.3390/cancers12010126

Figure Lengend Snippet: Validation of JAK1 variant by Sanger sequencing. ( A ) Sequencing chromatographs from Sanger sequencing validate JAK1 mutation in RNK-16 cell line. ( B ) JAK1 variant occurs in the kinase domain at Y1034, resulting in a change from tyrosine to cysteine. It sits within the activation loop, adjacent to another phosphorylation site at Y1033.

Article Snippet: Table S1: Alignment statistics for the RNK-16 and F344/NCrl samples, Table S2: Complete list of variants with an alternate allele in the RNK-16 cell line compared to F344/NCrl, Table S3: List of human homolog genes mapped to rat genes, with variants in RNK-16 cell line filtered by Q score and impact factor, Table S4: Primer design for WGS validation by Sanger sequencing.

Techniques: Biomarker Discovery, Variant Assay, Sequencing, Mutagenesis, Activation Assay, Phospho-proteomics

Journal: Cancers

Article Title: Whole Genome Sequencing of Spontaneously Occurring Rat Natural Killer Large Granular Lymphocyte Leukemia Identifies JAK1 Somatic Activating Mutation

doi: 10.3390/cancers12010126

Figure Lengend Snippet: In vivo and ex vivo characterization of RNK material. A total of 1 x 10 6 cells from RNK-1, -3, -7, and -16 were injected i.p. into young F344 rats and the time to disease was measured. ( A ) Sanger sequencing of primary spleen mononuclear cells harvested from leukemic animals showed a Jak 1 Y1034C mutation found only in RNK-16. ( B ) Those engrafted with RNK-16 had significantly shorter time to disease onset compared to the others. ( C ) Spleen cells were harvested from leukemic rats at euthanasia. Immunoblot analysis showed increased STAT1, -3, and -5 phosphorylation in RNK-16 relative to RNK-1, -3, and -7. More details of western blot, please view at the .

Article Snippet: Table S1: Alignment statistics for the RNK-16 and F344/NCrl samples, Table S2: Complete list of variants with an alternate allele in the RNK-16 cell line compared to F344/NCrl, Table S3: List of human homolog genes mapped to rat genes, with variants in RNK-16 cell line filtered by Q score and impact factor, Table S4: Primer design for WGS validation by Sanger sequencing.

Techniques: In Vivo, Ex Vivo, Injection, Sequencing, Mutagenesis, Western Blot, Phospho-proteomics

Journal: Advanced Science

Article Title: Efficient Biosynthetic Fabrication of Spidroins with High Spinning Performance

doi: 10.1002/advs.202400128

Figure Lengend Snippet: a) Solid powder of biomimetic spidroin. (i) Dissolved states of biomimetic spidroin in different solutions. From left to right, 1 mg of biomimetic spidroin is dissolved in 1 mL of pure water, 100 mg of biomimetic spidroin is dissolved in 500 µL of HFIP, and 1 mg of background Escherichia coli protein is dissolved in 1 mL of HFIP (ii). b) SEM image of nanospidroin fibers obtained by electrospinning (iii) and (iv). c) Physical image of a nanospidroin fiber membrane (v); schematic diagram of friction power generation of nanospidroin fiber (vi), (vii). Electric potential generated by rubbing a nanospidroin fiber membrane is recorded by an oscilloscope (viii). Charging of a 50 nF capacitor by nano spidroin fiber under slow and fast hand rubbing (ix). LEDs are lit up by electrical energy generated by rubbing nanospidroin fibers (x). Scale bar: 5 mm (i), 20 µm (iii), 2 µm (iv), 1 cm (v), 2 cm (viii).

Article Snippet: In this paper, a biomimetic spidroin sequence design with high yield and good spinning performance is reported, and a strategy for electrospinning to produce an artificial nanogenerator is explored.

Techniques: Membrane, Generated